Recombinant Human MARCH8 protein, His-tagged

• Cat.No.: MARCH8-3221H
• Product Overview: Recombinant Human MARCH8 protein(1-158 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-158 aa
• AA Sequence: MSMPLHQISAIPSQDAISARVYRSKTKEKEREEQNEKTLGHFMSHSSNISKAGSPPSASAPAPVSSFSRTSITPSSQDICRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYEFIMETKLKPLRKWEKLQMTSSERRKIMCS
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: MARCH8 membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase [ Homo sapiens ]
• Official Symbol: MARCH8
• Synonyms: MIR; c-MIR; RNF178; MARCH-VIII
• Gene ID: 220972
• mRNA Refseq: NM_145021.4
• Protein Refseq: NP_659458.2
• MIM: 613335
• UniProt ID: Q5T0T0