Recombinant Human MAP3K5, GST-tagged, Active

• Cat.No.: MAP3K5-245H
• Product Overview: Recombinant human ASK1(649-946) was expressed by baculovirus inSf9 insect cellsusing a N-terminal GST tag. MW = 60 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 649-946 a.a.
• Description: ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha. ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: MAP3K5 mitogen-activated protein kinase kinase kinase 5 [ Homo sapiens ]
• Synonyms: mitogen-activated protein kinase kinase kinase 5; ASK1; MEKK5; MAPKKK5; MAP3K5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; apoptosis signal regulating kinase 1; ASK-1; EC 2.7.11.25; OTTHUMP00000017275; Apoptosis signal-regulating kinase 1; MEK kinase 5
• Gene ID: 4217
• mRNA Refseq: NM_005923
• Protein Refseq: NP_005914
• MIM: 602448
• UniProt ID: Q99683
• Chromosome Location: 6q22.33
• Pathway: Amyotrophic lateral sclerosis (ALS); MAPK signaling pathway; Neurotrophin signaling pathway
• Function: MAP kinase kinase kinase activity; protein serine/threonine kinase activity; protein homodimerization activity; ATP binding; caspase activator activity; magnesium ion binding; nucleotide binding; transferase activity