Recombinant Human MAP/Microtubule Affinity-Regulating Kinase 4, GST-tagged

• Cat.No.: MARK4-1058H
• Product Overview: Recombinant full-length human MARK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: MARK4 or microtubule affinity-regulating kinase 4 is a member of the Par-1 family of serine/threonine protein kinases. MARK4 is predominantly expressed in the brain and readily phosphorylates Tau, MAP2 and MAP4. MARK4 co-localizes with the centrosome and microtubules in cultured cells. Overexpression of MARK4 causes thinning out of the microtubule network concomitant with the reorganization of microtubules into bundles. MARK4 provides a growth advantage to cells and the up-regulation of this kinase during focal ischaemia may represent an interesting new target for pharmacological intervention. MARK4 is expressed during the cell cycle and is linked to aberrant centrosomes in glioma cells.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 104 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Specific Activity: 1050 nmol/min/mg
• Purity: > 90%
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: LKB1 signaling events

Gene Information

• Gene Name: MARK4 MAP/microtubule affinity-regulating kinase 4 [ Homo sapiens ]
• Official Symbol: MARK4
• Synonyms: MARK4; MAP/microtubule affinity-regulating kinase 4; MARK4L; MARK4S; MARKL1; MARKL1L; FLJ12177; FLJ90097; KIAA1860; MAP/microtubule affinity-regulating kinase 4; MARK4 serine/threonine protein kinase; MAP/microtubule affinity-regulating kinase like 1; EC 2.7.11.1
• Gene ID: 57787
• mRNA Refseq: NM_031417
• Protein Refseq: NP_000446
• MIM: 606495
• UniProt ID: Q96L34
• Chromosome Location: 19q13.3
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; tau-protein kinase activity; ubiquitin binding; microtubule binding; gamma-tubulin binding; transferase activity, transferring phosphorus-containing groups