Recombinant Human mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, His-tagged

• Cat.No.: MGAT2-517H
• Product Overview: MGAT2, 30-447aa Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 30-447 a.a.
• Description: MGAT2 is a golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Transcript variants with a spliced 5" UTR may exist, but their biological validity has not been determined. Recombinant human MGAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 50 kDa (439aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MRQRKNEALA PPLLDAEPAR GAGGRGGDHP SVAVGIRRVS NVSAASLVPA VPQPEADNLT LRYRSLVYQL NFDQTLRNVD KAGTWAPREL VLVVQVHNRP EYLRLLLDSL RKAQGIDNVL VIFSHDFWST EINQLIAGVN FCPVLQVFFP FSIQLYPNEF PGSDPRDCPR DLPKNAALKL GCINAEYPDS FGHYREAKFS QTKHHWWWKL HFVWERVKIL RDYAGLILFL EEDHYLAPDF YHVFKKMWKL KQQECPECDV LSLGTYSASR SFYGMADKVD VKTWKSTEHN MGLALTRNAY QKLIECTDTF CTYDDYNWDW TLQYLTVSCL PKFWKVLVPQ IPRIFHAGDC GMHHKKTCRP STQSAQIESL LNNNKQYMFP ETLTISEKFT VVAISPPRKN GGWGDIRDHE LCKSYRRLQ
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MGAT2?mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [?Homo sapiens?(human) ]
• Official Symbol: MGAT2
• Synonyms: MGAT2; GNT2; CDG2A; CDGS2; GNT-II; GLCNACTII; mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase; alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase; glcNAc-T II; mannoside acetylglucosaminyltransferase 2; Beta-1,2-N-acetylglucosaminyltransferase II; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II; UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II; NP_002399.1; EC 2.4.1.143
• Gene ID: 4247
• mRNA Refseq: NM_002408
• Protein Refseq: NP_002399
• MIM: 602616
• UniProt ID: Q10469
• Chromosome Location: 14q21
• Pathway: Asparagine N-linked glycosylation; N-Glycan biosynthesis; Reactions specific to the complex N-glycan synthesis pathway
• Function: alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity; carbohydrate binding