Recombinant Human MAGEA4 protein(1-317aa), His-tagged

• Cat.No.: MAGEA4-8754H
• Product Overview: Recombinant Human MAGEA4 protein(P43358)(1-317aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-317aa
• Form: Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human MAGEA4 at 2 μg/Ml can bind anti-MAGEA4 recombinant antibody, the EC50 is 30.33-43.10 ng/mL.
• Molecular Mass: 36.7 kDa
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Purity: Greater than 90% as determined by SDS-PAGE.
  Greater than 90% as determined by SEC-HPLC.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.
• AA Sequence: MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPASNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV

Gene Information

• Gene Name: MAGEA4 melanoma antigen family A, 4 [ Homo sapiens ]
• Official Symbol: MAGEA4
• Synonyms: MAGEA4; melanoma antigen family A, 4; MAGE4; melanoma-associated antigen 4; cancer/testis antigen family 1; member 4; CT1.4; MAGE 41; MAGE X2; MAGE4A; MAGE4B; melanoma associated antigen 4; MGC21336; MAGE-4 antigen; MAGE-41 antigen; MAGE-X2 antigen; cancer/testis antigen 1.4; cancer/testis antigen family 1, member 4; MAGE-41; MAGE-X2
• Gene ID: 4103
• mRNA Refseq: NM_001011548
• Protein Refseq: NP_001011548
• MIM: 300175
• UniProt ID: P43358