Recombinant Human MAG protein, His-tagged

• Cat.No.: MAG-6985H
• Product Overview: Recombinant Human MAG protein(P20916)(Gly20-Pro516), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gly20-Pro516
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in PBS, 100mM L-Arginine (pH 7.4).
• Bio-activity: Immobilized Human Siglec-4a, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 55.8 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MAG myelin associated glycoprotein [ Homo sapiens ]
• Official Symbol: MAG
• Synonyms: MAG; myelin associated glycoprotein; GMA; myelin-associated glycoprotein; S MAG; sialic acid binding Ig like lectin 4A; SIGLEC 4A; SIGLEC4A; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A; S-MAG; SIGLEC-4A
• Gene ID: 4099
• mRNA Refseq: NM_001199216
• Protein Refseq: NP_001186145
• MIM: 159460
• UniProt ID: P20916