Recombinant Human LRRC32 & TGFB1 protein, His-Avi-tagged

• Cat.No.: LRRC32 & TGFB1-2140H
• Product Overview: Recombinant Human LRRC32 & TGFB1 protein(Q14392(GARP(G139N))&P01137(Latent TGF beta 1))(His20-Leu628(LRRC32(G139N)) & Leu30-Ser390(TGFB1)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: His20-Leu628(LRRC32(G139N)) & Leu30-Ser390(TGFB1)
• Tag: C-His-Avi
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Anti-GARP&TGF beta 1 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for GARP (G139N) &Latent TGF Beta 1 Complex, His Tag with the EC50 of 1.05μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 70.3 kDa (GARP(G139N))&41.4 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 75-80 kDa (GARP(G139N))&15 kDa&42-48 kDa (Latent TGF Beta 1) based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• GeneID 2: 7040

Gene Information

• Gene Name: LRRC32 leucine rich repeat containing 32 [ Homo sapiens ]
• Official Symbol: LRRC32
• Synonyms: LRRC32; leucine rich repeat containing 32; D11S833E, GARP, glycoprotein A repetitions predominant; leucine-rich repeat-containing protein 32; garpin; glycoprotein A repetitions predominant; GARP; D11S833E
• Gene ID: 2615
• mRNA Refseq: NM_001128922
• Protein Refseq: NP_001122394
• MIM: 137207
• UniProt ID: Q14392
• Gene Name 2: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol 2: TGFB1
• Synonyms 2: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• mRNA Refseq 2: NM_000660
• Protein Refseq 2: NP_000651
• MIM 2: 190180
• UniProt ID 2: P01137