Recombinant Human LMAN1 protein, His-tagged

• Cat.No.: LMAN1-7896H
• Product Overview: Recombinant Human LMAN1 aa. (Pro198~Cys475) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Pro198~Cys475
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 5.3.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: LMAN1 lectin, mannose binding 1 [ Homo sapiens (human) ]
• Official Symbol: LMAN1
• Synonyms: LMAN1; lectin, mannose binding 1; MR60; gp58; F5F8D; FMFD1; MCFD1; ERGIC53; ERGIC-53; protein ERGIC-53; ER-Golgi intermediate compartment 53 kDa protein; endoplasmic reticulum-golgi intermediate compartment protein 53; intracellular mannose-specific lectin MR60
• Gene ID: 3998
• mRNA Refseq: NM_005570.3
• Protein Refseq: NP_005561.1
• UniProt ID: P49257