Recombinant Human LIPC, His-tagged

• Cat.No.: LIPC-268H
• Product Overview: Recombinant Human LIPC(Ser347~Pro478), fused with N-terminal His-Tag, was expressed in E.coli.

Specification

• Description: LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 16.7kDa
• AA Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.MGHHHHHHSGSEF- SPFK VYHYQFKIQF INQTETPIQT TFTMSLLGTK EKMQKIPITL GKGIASNKTY SFLITLDVDI GELIMIKFKW ENSAVWANVW DTVQTIIPWS TGPRHSGLVL KTIRVKAGET QQRMTFCSEN TDDLLLRP
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Protein length: 347-478 a.a.

Gene Information

• Gene Name: LIPC lipase, hepatic [ Homo sapiens (human) ]
• Official Symbol: LIPC
• Synonyms: LIPC; lipase, hepatic; HL; HTGL; LIPH; HDLCQ12; hepatic triacylglycerol lipase; hepatic lipase; lipase member C; NP_000227.2; EC 3.1.1.3
• Gene ID: 3990
• mRNA Refseq: NM_000236
• Protein Refseq: NP_000227
• MIM: 151670
• UniProt ID: P11150
• Chromosome Location: 15q21-q23
• Pathway: Acylglycerol degradation; Fatty Acid Beta Oxidation; Lipoprotein metabolism
• Function: apolipoprotein binding; low-density lipoprotein particle binding; triglyceride lipase activity