Recombinant Human Lin-28 Homolog B (C. elegans), His-tagged

• Cat.No.: LIN28B-7186H
• Product Overview: Recombinant human Lin28B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 29.5 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-250aa
• Description: Lin28B belongs to the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28B is acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 10% glycerol,2mM DTT.
• Molecular Mass: 29.5 kDa (273aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: LIN28B lin-28 homolog B (C. elegans) [ Homo sapiens ]
• Official Symbol: LIN28B
• Synonyms: LIN28B; lin-28 homolog B (C. elegans); protein lin-28 homolog B; CSDD2; FLJ16517; lin-28B; Lin-28.2
• Gene ID: 389421
• mRNA Refseq: NM_001004317
• Protein Refseq: NP_001004317
• MIM: 611044
• UniProt ID: Q6ZN17
• Chromosome Location: 6q21
• Pathway: Validated targets of C-MYC transcriptional activation, organism-specific biosystem
• Function: DNA binding; RNA binding; metal ion binding; protein binding; zinc ion binding