Recombinant Human LIMK2 Protein, GST/His-tagged

• Cat.No.: LIMK2-382H
• Product Overview: Recombinant Human LIMK2, transcript variant 1, fused with N-terminal GST and C-terminal His was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&GST
• Description: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
• Form: 25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.
• Molecular Mass: 103.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: LIMK2 LIM domain kinase 2 [ Homo sapiens ]
• Official Symbol: LIMK2
• Synonyms: LIMK2; LIM domain kinase 2
• Gene ID: 3985
• mRNA Refseq: NM_001031801
• Protein Refseq: NP_001026971
• MIM: 601988
• UniProt ID: P53671