Recombinant Human LIMK1

• Cat.No.: LIMK1-29945TH
• Product Overview: Recombinant fragment, corresponding to amino acids 285-638 of Human LIM Kinase 1 was expressed by baculovirus in Sf9 cells using an N-terminal tag, 41kDa.

Specification

• Species: Human
• Tag: Non
• Protein Length: 285-638 a.a.
• Description: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Tissue specificity: Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
• Biological activity: This is an inactive protein.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.Contains 2 LIM zinc-binding domains.Contains 1 PDZ (DHR) domain.Contains 1 protein kinase domain.

Gene Information

• Gene Name: LIMK1 LIM domain kinase 1 [ Homo sapiens ]
• Official Symbol: LIMK1
• Synonyms: LIMK1; LIM domain kinase 1; LIMK
• Gene ID: 3984
• mRNA Refseq: NM_001204426
• Protein Refseq: NP_001191355
• MIM: 601329
• Uniprot ID: P53667
• Chromosome Location: 7q11.23
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem
• Function: ATP binding; heat shock protein binding; metal ion binding; nucleotide binding; protein binding