Recombinant Human LIGHT protein(Asp74-Val240), Avi&Fc-tagged, Biotinylated

• Cat.No.: LIGHT-2390H
• Product Overview: Recombinant Human TNFSF14 (NP_001363816.1) (Asp74-Val240) was expressed in HEK293 with a N-terminal AVI tag followed by a Fc region of Human IgG1 tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Specification

• Source: HEK293
• Species: Human
• Tag: N-Avi&Fc
• Protein length: Asp74-Val240
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human HVEM His at 2 μg/mL (100 μL/well) can bind Human LIGHT (ECD, hFc & AVI Tag), Biotinylated, the EC50 is 20-180 ng/mL.
• Molecular Mass: The recombinant human TNFSF14 consists of 420 amino acids and predicts a molecular mass of 46.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.