Recombinant Human LIAS Protein, His-tagged

• Cat.No.: LIAS-2393H
• Product Overview: Recombinant Human LIAS Protein (Met1-Gln288) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. Localized in the mitochondrion, this iron-sulfur enzyme catalyzes the final step in the de novo pathway for the biosynthesis of lipoic acid, a potent antioxidant. The deficient expression of this enzyme has been linked to conditions such as diabetes, atherosclerosis and neonatal-onset epilepsy. Alternative splicing occurs at this locus, and several transcript variants encoding distinct isoforms have been identified.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 35.8 kDa
  Accurate Molecular Mass: 36 kDa
• Protein length: Met1-Gln288
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: LIAS lipoic acid synthetase [ Homo sapiens (human) ]
• Official Symbol: LIAS
• Synonyms: LIAS; lipoic acid synthetase; lipoyl synthase, mitochondrial; LAS; lip-syn; lipoate synthase; LS; LIP1; PDHLD; HUSSY-01; MGC23245
• Gene ID: 11019
• mRNA Refseq: NM_006859
• Protein Refseq: NP_006850
• MIM: 607031
• UniProt ID: O43766