Recombinant Human LGALS8

• Cat.No.: LGALS8-3624H
• Product Overview: Recombinant Human Galectin-8 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 317 amino acids and having a molecular mass of 35.8 kDa.

Specification

• Cat. No.: LGALS8-3624H
• Description: Galectins are a family of animal lectins with an affinity for β-galactosides. There are at least 14 members identified. Galectins share similarities in carbohydrate recognition domain (CRD). Galectins are synthesized as cytosolic proteins. Although localized primarily in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are implicated in modulating cell-cell and cell-matrix interactions. Galectin-8 is a tandem-repeat-type member of galectin family, consisting of two CRDs joined by a linker peptide. Galectin-8 is highly expressed in lung carcinomas,certain forms of prostate carcinomas, as well as other tumor cells. It is also known as prostate carcinoma tumor antigen 1 (PCTA1) in human. Galectin-8 binds to a subset of cell surface integrins to modulate ECM-integrin interactions. When immobilized, Galectin-8 promotes cell adhesion by ligation and clustering of cell surface integrin receptors. In contrast, as a soluble ligand, Galectin-8 can inhibit cell adhesion. Human and mouse Galectin-8 share 80% sequence identity.
• Biological Activity: The ED50 of rHuGalectin-8 as determined by its ability to agglutinate human red blood cells is 0.8~4 μg/ml.
• Purity: Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Met-Leu-Ser-Leu.
• Endotoxin: Less than 0.1ng/μg (1.0EU/μg) of rHuGalectin-8 as determined by LAL test.
• Form: The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuGalectin-8 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Lyophilized rHuGalectin-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuGalectin-8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: LGALS8 lectin, galactoside-binding, soluble, 8 [ Homo sapiens ]
• Official Symbol: LGALS8
• Synonyms: LGALS8;Gal-8; PCTA1; PCTA-1; Po66-CBP; galectin-8; galectin 8; galectin-8g; OTTHUMP00000037853; OTTHUMP00000037854; OTTHUMP00000037855; OTTHUMP00000037957; OTTHUMP00000037958; OTTHUMP00000037959; OTTHUMP00000037961; OTTHUMP00000038817; OTTHUMP00000231517; OTTHUMP00000231546; Po66 carbohydrate binding protein; po66 carbohydrate-binding protein; prostate carcinoma tumor antigen 1; Prostate carcinoma tumor antigen 1
• Gene ID: 3964
• mRNA Refseq: NM_006499
• Protein Refseq: NP_006490
• MIM: 606099
• UniProt ID: O00214
• Chromosome Location: 1q43
• Function: sugar binding