Recombinant Human leukocyte-associated immunoglobulin-like receptor 1 Protein, His&Flag&StrepII tagged

• Cat.No.: LAIR1-037H
• Product Overview: Recombinant human LAIR1 protein (Q6GTX8) (His 187-His 287) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Description: Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
• Source: E. coli
• Species: Human
• Tag: N-Twin strep and C-His&Flag
• Protein length: 187-287aa
• Molecular Mass: 17.8 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: LAIR1 leukocyte-associated immunoglobulin-like receptor 1 [ Homo sapiens (human) ]
• Official Symbol: LAIR1
• Synonyms: LAIR1; leukocyte-associated immunoglobulin-like receptor 1; leukocyte associated Ig like receptor 1; CD305; hLAIR1; leukocyte-associated Ig-like receptor 1; LAIR-1
• Gene ID: 3903
• mRNA Refseq: NM_002287
• Protein Refseq: NP_002278
• MIM: 602992
• UniProt ID: Q6GTX8