Recombinant Human LEPR protein, His-tagged

• Cat.No.: LEPR-515H
• Product Overview: Recombinant Human LEPR (P48357) aa. (Phe22~Asp839) fused with His tag was produced in HEK293F cells.

Specification

• Description: The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins
• Source: HEK293F
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 95.1kDa; Accurate Molecular Mass: 95kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Phe22~Asp839
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 97%
• Characteristic: Isoelectric point is 7.2.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: LEPR leptin receptor [ Homo sapiens (human) ]
• Official Symbol: LEPR
• Synonyms: LEPR; OBR; OB-R; CD295; LEP-R; LEPRD; leptin receptor; OB receptor; huB219
• Gene ID: 3953
• mRNA Refseq: NM_001003679.3
• Protein Refseq: NP_001003679.1
• UniProt ID: P48357