Recombinant Human Lck interacting transmembrane adaptor 1, His-tagged

• Cat.No.: LIME1-323H
• Product Overview: LIME1, 28-295aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 28-295 a.a.
• Description: Lck-interacting transmembrane adapter 1 precursor, also known as LIME1, was previously characterized as a transmembrane adaptor protein mediating TCR-dependent T cell activation. LIME1 becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME1 associates with the Src family kinase Lck and its negative regulator, Csk. Recombinant human LIME1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
• Molecular Mass: 30.8 kDa (291aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSCTACRRP EDAVAPRKRA RRQRARLQGS ATAAEASLLR RTHLCSLSKS DTRLHELHRG PRSSRALRPA SMDLLRPHWL EVSRDITGPQ AAPSAFPHQE LPRALPAAAA TAGCAGLEAT YSNVGLAALP GVSLAASPVV AEYARVQKRK GTHRSPQEPQ QGKTEVTPAA QVDVLYSRVC KPKRRDPGPT TDPLDPKGQG AILALAGDLA YQTLPLRALD VDSGPLENVY ESIRELGDPA GRSSTCGAGT PPASSCPSLG RGWRPLPASL P
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: LIME1 Lck interacting transmembrane adaptor 1 [ Homo sapiens ]
• Official Symbol: LIME1
• Synonyms: LIME1; Lck interacting transmembrane adaptor 1; lck-interacting transmembrane adapter 1; dJ583P15.4; FLJ20406; LIME; lck-interacting molecule; lck-interacting membrane protein; LP8067; RP4-583P15.5
• Gene ID: 54923
• mRNA Refseq: NM_017806
• Protein Refseq: NP_060276
• MIM: 609809
• UniProt ID: Q9H400
• Chromosome Location: 20q13.33
• Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem
• Function: growth factor receptor binding; protein kinase binding