Recombinant Human LCAT, Flag tagged

• Cat.No.: LCAT-8326H
• Product Overview: LCAT Human Recombinant produced in HEK is a single, glycosylated, polypeptide chain containing 429 amino acids (25-440) which includes 13 amino acid Flag Tag fused at N-terminus and having total molecular mass of 48.5 kDa. LCAT Human Recombinant is purified by chromatographic techniques.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 25-440 a.a.
• Description: This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency.
• Form: The LCAT protein was lyophilized from 0.4 um filtered solution at concentration of 0.5mg/ml containing 20 mM Tris buffer, and 50mM NaCl, pH 7.5.
• AA Sequence: HVDYKDDDDK PAGFWLLNVL FPPHTTPKAE LSNHTRPVIL VPGCLGNQLE AKLDKPDVVN WMCYRKTEDF FTIWLDLNMF LPLGVDCWID NTRVVYNRSS GLVSNAPGVQ IRVPGFGKTY SVEYLDSSKL AGYLHTLVQN LVNNGYVRDE TVRAAPYDWR LEPGQQEEYY RKLAGLVEEM HAAYGKPVFL IGHSLGCLHL LYFLLRQPQA WKDRFIDGFI SLGAPWGGSI KPMLVLASGD NQGIPIMSSI KLKEEQRITT TSPWMFPSRM AWPEDHVFIS TPSFNYTGRD FQRFFADLHF EEGWYMWLQS RDLLAGLPAP GVEVYCLYGV GLPTPRTYIY DHGFPYTDPV GVLYEDGDDT VATRSTELCG LWQGRQPQPV HLLPLHGIQH LNMVFSNLTL EHINAILLGA YRQGPPASPT ASPEPPPPE
• Notes: For research use only.
• Storage: Lyophilized LCAT although stable at room temperature for weeks, should be stored desiccated below -18 ℃. Upon reconstitution LCAT should be stored at 4 ℃ between 2-7 days and for future use below-18 ℃. For long term storage it is recommended to add carrie
• Reconstitution: It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: LCAT lecithin-cholesterol acyltransferase [ Homo sapiens ]
• Official Symbol: LCAT
• Synonyms: LCAT; lecithin-cholesterol acyltransferase; phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase
• Gene ID: 3931
• mRNA Refseq: NM_000229
• Protein Refseq: NP_000220
• MIM: 606967
• UniProt ID: P04180
• Chromosome Location: 16q22.1
• Pathway: Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; HDL-mediated lipid transport, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem
• Function: apolipoprotein A-I binding; phosphatidylcholine-sterol O-acyltransferase activity; phosphatidylcholine-sterol O-acyltransferase activity; phospholipase A2 activity; protein binding; transferase activity, transferring acyl groups