Recombinant Human LCAT, Flag-tagged

• Cat.No.: LCAT-1013H
• Product Overview: Total 429 AA. MW: 48.5 kDa (calculated). UniProtKB/Swiss-Prot P04180 Phe25-Glu440 with N-Terminal Flag-tag, 13 extra AA (highlighted).

Specification

• Cat. No.: LCAT-1013H
• Description: LCAT is an enzyme that converts free cholesterol into cholesteryl ester (a more hydrophobic form of cholesterol), which is then sequestered into the core of a lipoprotein particle, eventually making the newly synthesized HDL spherical and forcing the reaction to become unidirectional since the particles are removed from the surface. The enzyme is bound to high-density lipoproteins (HDLs) and low-density lipoproteins in the blood plasma.
• Source: HEK293
• Species: Human
• Form: Filtered (0. 4 μm) and lyophilized from 0.5 mg/ml in 20 mM Tris buffer, 50 mM NaCl, Ph 7.5
• Research topic: Energy metabolism and body weight regulation
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: HVDYKDDDDK PAGFWLLNVL FPPHTTPKAE LSNHTRPVIL VPGCLGNQLE AKLDKPDVVN WMCYRKTEDF FTIWLDLNMF LPLGVDCWID NTRVVYNRSS GLVSNAPGVQ IRVPGFGKTY SVEYLDSSKL AGYLHTLVQN LVNNGYVRDE TVRAAPYDWR LEPGQQEEYY RKLAGLVEEM HAAYGKPVFL IGHSLGCLHL LYFLLRQPQA WKDRFIDGFI SLGAPWGGSI KPMLVLASGD NQGIPIMSSI KLKEEQRITT TSPWMFPSRM AWPEDHVFIS TPSFNYTGRD FQRFFADLHF EEGWYMWLQS RDLLAGLPAP GVEVYCLYGV GLPTPRTYIY DHGFPYTDPV GVLYEDGDDT VATRSTELCG LWQGRQPQPV HLLPLHGIQH LNMVFSNLTL EHINAILLGA YRQGPPASPT ASPEPPPPE
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: LCAT lecithin-cholesterol acyltransferase [ Homo sapiens ]
• Official Symbol: LCAT
• Synonyms: LCAT; lecithin-cholesterol acyltransferase; phosphatidylcholine-sterol acyltransferase; OTTHUMP00000174856; phospholipid-cholesterol acyltransferase
• Gene ID: 3931
• mRNA Refseq: NM_000229
• Protein Refseq: NP_000220
• MIM: 606967
• UniProt ID: P04180
• Chromosome Location: 16q22
• Pathway: Glycerophospholipid metabolism; HDL-mediated lipid transport; Lipid digestion; Lipoprotein metabolism; Metabolism of lipids and lipoproteins; Statin Pathway
• Function: apolipoprotein A-I binding; phosphatidylcholine-sterol O-acyltransferase activity; phospholipase A2 activity; protein binding; transferase activity