Recombinant Human LAMA1 protein, His-tagged

• Cat.No.: LAMA1-2590H
• Product Overview: Recombinant Human LAMA1 aa. (Val2613~Phe2746) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Val2613~Phe2746
• Description: This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome.
• Form: Freeze-dried powder
• Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: LAMA1 laminin subunit alpha 1 [ Homo sapiens (human) ]
• Official Symbol: LAMA1
• Synonyms: LAMA1; laminin subunit alpha 1; LAMA; PTBHS; S-LAM-alpha; laminin subunit alpha-1; S-LAM alpha; S-laminin subunit alpha; laminin A chain; laminin, alpha 1; laminin-3 subunit alpha
• Gene ID: 284217
• mRNA Refseq: NM_005559.3
• Protein Refseq: NP_005550.2
• UniProt ID: P25391