Recombinant Human LAIR1 protein, Fc-tagged

• Cat.No.: LAIR1-377H
• Product Overview: Recombinant Human LAIR1 protein (Gln22-His163), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is constitutively expressed on the majority of human peripheral blood mononuclear leukocytes. LAIR-1 or CD305 is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs. LAIR-1 recruits SHP-1 and SHP-2 phosphatases upon activation, and cross-linking of the LAIR-1 antigen on natural killer (NK) cells results in strong inhibition of NK cell-mediated cytotoxicity.
  Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation.Diseases associated with LAIR1 include Chronic Active Epstein-Barr Virus Infection and Palindromic Rheumatism.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 42.1 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 142
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: LAIR1
• Official Symbol: LAIR1
• Synonyms: LAIR1; leukocyte-associated immunoglobulin-like receptor 1; leukocyte associated Ig like receptor 1; CD305; hLAIR1; leukocyte-associated Ig-like receptor 1; LAIR-1
• Gene ID: 3903
• mRNA Refseq: NM_002287
• Protein Refseq: NP_002278
• MIM: 602992
• UniProt ID: Q6GTX8