Recombinant Human KRT2 protein, His-tagged

• Cat.No.: KRT2-2697H
• Product Overview: Recombinant Human KRT2 aa. (Cys7~Leu160 (Accession # P35908)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Cys7~Leu160
• Description: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 15.0kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 11.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: KRT2 keratin 2 [ Homo sapiens (human) ]
• Official Symbol: KRT2
• Synonyms: K2e; KRTE; CK-2e; KRT2A; KRT2E; cytokeratin-2e; epithelial keratin-2e; keratin 2, type II; keratin-2 epidermis; keratin-2e; type-II keratin Kb2; keratin, type II cytoskeletal 2 epidermal
• Gene ID: 3849
• mRNA Refseq: NM_000423.2
• Protein Refseq: NP_000414.2
• UniProt ID: P35908