Recombinant Human KRT14 protein, His & T7-tagged

• Cat.No.: KRT14-2729H
• Product Overview: Recombinant Human KRT14 aa. (Leu111~Leu418 (Accession # P02533)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Leu111~Leu418
• Description: This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 39.7kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.1.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: KRT14 keratin 14 [ Homo sapiens (human) ]
• Official Symbol: KRT14
• Synonyms: K14; NFJ; CK14; EBS3; EBS4; cytokeratin 14; keratin 14, type I; keratin, type I cytoskeletal 14
• Gene ID: 3861
• mRNA Refseq: NM_000526.4
• Protein Refseq: NP_000517.2
• UniProt ID: P02533