Recombinant Human KRT10 protein, His-tagged

• Cat.No.: KRT10-010H
• Product Overview: Recombinant Human KRT10 (Leu153~Glu456) was expressed in E. coli with N-terminal His Tag.

Specification

• Description: This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 36.6kDa
• Endotoxin: < 1.0EU per 1μg (determined by the LAL method)
• Purity: > 97% as determined by SDS-PAGE and Coomassie blue staining
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 °C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Protein length: 153-456 a.a.

Gene Information

• Gene Name: KRT10 keratin 10 [ Homo sapiens (human) ]
• Official Symbol: KRT10
• Synonyms: BIE; EHK; K10; KPP; BCIE; CK10
• Gene ID: 3858
• mRNA Refseq: NM_000421.3
• Protein Refseq: NP_000412.3
• MIM: 148080
• UniProt ID: P13645