Recombinant Human KRAS G12C Protein, AviTag™, Biotinylated

• Cat.No.: KRAS-09H
• Product Overview: Recombinant human KRAS G12C protein (amino acids 1-185; G12C mutant variant of isoform 2B) with the N-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme.

Specification

• Species: Human
• Source: E.coli
• Tag: Avi
• Protein Length: 1-185
• Description: Human KRAS protein is a small guanosine triphosphatase (GTPase) that is the component of the mitogen activated protein kinase (MAPK) signaling pathway. High occurrences of KRAS mutations in some types of cancers make KRAS one of the most important targets in oncology for drug development. The four most frequent KRAS mutations are G12D, G12V, G13D, and G12C.
• AA Sequence: MGSHHHHHHHHSNGLNDIFEAQKIEWHEMTEYKLVVVGACGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKC
• Purity: > 90% by Coomassie staining
• Notes: This product is for laboratory research use only.
• Storage: Storage is recommended at -20 centigrade for longer periods of time.
• Concentration: 1.0 mg/mL by A280
• Storage Buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol.
• Shipping: Product requires shipping on ice packs.

Gene Information

• Gene Name: KRAS KRAS proto-oncogene, GTPase [ Homo sapiens (human) ]
• Official Symbol: KRAS
• Synonyms: KRAS; KRAS proto-oncogene, GTPase; NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; 'C-K-RAS; c-Ki-ras; c-Ki-ras2; GTPase KRas; K-ras p21 protein; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral proto-oncogene; PR310 c-K-ras oncogene; c-Kirsten-ras protein; cellular c-Ki-ras2 proto-oncogene; cellular transforming proto-oncogene; oncogene KRAS2; proto-oncogene GTPase; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; EC 3.6.5.10; KRAS G12C
• Gene ID: 3845
• mRNA Refseq: NM_033360
• Protein Refseq: NP_203524
• MIM: 190070
• UniProt ID: P01116