Recombinant Human KLRK1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: KLRK1-718HB
• Product Overview: Biotinylated Recombinant Human KLRK1 protein(P26718)(Phe78-Val216), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-His-Avi
• Protein length: Phe78-Val216
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human NKG2D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human MICA, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLRK1 killer cell lectin-like receptor subfamily K, member 1 [ Homo sapiens ]
• Official Symbol: KLRK1
• Synonyms: KLRK1; killer cell lectin-like receptor subfamily K, member 1; D12S2489E, DNA segment on chromosome 12 (unique) 2489 expressed sequence; NKG2-D type II integral membrane protein; CD314; KLR; NKG2 D; NKG2D; NK cell receptor D; NKG2-D-activating NK receptor; NKG2-D; D12S2489E; FLJ17759; FLJ75772
• Gene ID: 22914
• mRNA Refseq: NM_007360
• Protein Refseq: NP_031386
• MIM: 611817
• UniProt ID: P26718