Recombinant Human KLF4, His-tagged

• Cat.No.: KLF4-8466H
• Product Overview: Recombinant Human KLF4, fused with His tag, was expressed in E. coli and purified by affinity chromatography in combination with FPLC columns.

Specification

• Description: In embryonic stem cells (ESCs). KLF4 has been demonstrated to be a good indicator of stem-like capacity. As a transcription factor. it can act both as activator and as repressor. KLF4 binds the 5"-CACCC-3" core sequence and regulates the expression of key transcription factors during embryonic development. It plays an important role in maintaining embryonic stem cells. and in preventing their differentiation. KLF4 also contributes to the down-regulation of p53/TP53 transcription.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol. 100 mM KCl. 1 mM DTT and 0.2 mM EDTA
• Molecular Mass: 51.1 kDa
• Endotoxin: Less than 1.0 EU/μg by LAL method
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Shipping: Dry Ice

Gene Information

• Gene Name: KLF4 Kruppel-like factor 4 (gut) [ Homo sapiens ]
• Official Symbol: KLF4
• Synonyms: KLF4; Kruppel-like factor 4 (gut); Krueppel-like factor 4; EZF; GKLF; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein
• Gene ID: 9314
• mRNA Refseq: NM_004235
• Protein Refseq: NP_004226
• MIM: 602253
• UniProt ID: O43474
• Chromosome Location: 9q31
• Pathway: Developmental Biology, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Synthesis, Secretion, and Deacylation of Ghrelin, organism-specific biosystem; Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem
• Function: DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; core promoter proximal region sequence-specific DNA binding; double-stranded DNA binding; metal ion binding; phosphatidylinositol 3-kinase regulator activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor recruiting transcription factor activity; sequence-specific DNA binding transcription factor recruiting transcription factor activity; transcription regulatory region DNA binding; transcription regulatory region DNA binding; zinc ion binding