Recombinant Human KIR3DL3 protein, His-Avi-tagged

• Cat.No.: KIR3DL3-743H
• Product Overview: Recombinant Human KIR3DL3 protein(Q8N743)(Glu26-Leu322), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His-Avi
• Protein length: Glu26-Leu322
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 0.2M L-Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.