Recombinant Human kinesin family member 5B Protein, His&Twin Strep tagged

• Cat.No.: KIF5B-108H
• Product Overview: Recombinant human KIF5B protein (P33176) (Met 1-Thr328) with a Twin strep tag at the N-terminus and a His tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&Twin Strep
• Protein Length: 1-328aa
• Description: Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation. Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends. Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells.
• Tag: N-Twin strep and C-His
• Molecular Mass: 42 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: KIF5B kinesin family member 5B [ Homo sapiens (human) ]
• Official Symbol: KIF5B
• Synonyms: KIF5B; kinesin family member 5B; KNS1; kinesin-1 heavy chain; KNS; kinesin heavy chain; kinesin 1 (110-120kD); ubiquitous kinesin heavy chain; conventional kinesin heavy chain; KINH; UKHC
• Gene ID: 3799
• mRNA Refseq: NM_004521
• Protein Refseq: NP_004512
• MIM: 602809
• UniProt ID: P33176