Recombinant Human KCTD6 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: KCTD6-870H
• Product Overview: KCTD6 MS Standard C13 and N15-labeled recombinant protein (NP_699162) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Probable substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes the ubiquitination of HDAC1; the function seems to depend on KCTD11:KCTD6 oligomerization. Can function as antagonist of the Hedgehog pathway by affecting the nuclear transfer of transcription factor GLI1; the function probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. Inhibits cell growth and tumorigenicity of medulloblastoma (MDB). Involved in regulating protein levels of ANK1 isoform Mu17 probably implicating CUL3-dependent proteasomal degradation.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 27.6 kDa
• AA Sequence: MDNGDWGYMMTDPVTLNVGGHLYTTSLTTLTRYPDSMLGAMFGGDFPTARDPQGNYFIDRDGPLFRYVLNFLRTSELTLPLDFKEFDLLRKEADFYQIEPLIQCLNDPKPLYPMDTFEEVVELSSTRKLSKYSNPVAVIITQLTITTKVHSLLEGISNYFTKWNKHMMDTRDCQVSFTFGPCDYHQEVSLRVHLMEYITKQGFTIRNTRVHHMSERANENTVEHNWTFCRLARKTDDSGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: KCTD6 potassium channel tetramerisation domain containing 6 [ Homo sapiens (human) ]
• Official Symbol: KCTD6
• Synonyms: KCTD6; potassium channel tetramerisation domain containing 6; BTB/POZ domain-containing protein KCTD6; KCASH3; MGC27385
• Gene ID: 200845
• mRNA Refseq: NM_153331
• Protein Refseq: NP_699162
• MIM: 618791
• UniProt ID: Q8NC69