Recombinant Human KCTD17 293 Cell Lysate

Description :	Antigen standard for potassium channel tetramerisation domain containing 17 (KCTD17) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	KCTD17 potassium channel tetramerisation domain containing 17 [ Homo sapiens ]
Official Symbol	KCTD17
Synonyms	KCTD17; potassium channel tetramerisation domain containing 17; BTB/POZ domain-containing protein KCTD17; FLJ12242; FLJ98761;
Gene ID	79734
mRNA Refseq	NM_024681
Protein Refseq	NP_078957
UniProt ID	Q8N5Z5
Chromosome Location	22q12.3
Function	identical protein binding; voltage-gated potassium channel activity;