Recombinant Human KCNN2 Protein, His-tagged

• Cat.No.: KCNN2-2357H
• Product Overview: Recombinant Human KCNN2 Protein (Lys466-Ser579) with a N-His tag was expressed in E. coli.

Specification

• Description: Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene is a member of the KCNN family of potassium channel genes. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. Alternate splicing of this gene results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.8 kDa
  Accurate Molecular Mass: 17&20 kDa
• Protein length: Lys466-Ser579
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: KCNN2 potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 [ Homo sapiens (human) ]
• Official Symbol: KCNN2
• Synonyms: KCNN2; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2; small conductance calcium-activated potassium channel protein 2; hSK2; KCa2.2; SKCa 2; apamin-sensitive small-conductance Ca2+-activated potassium channel; SK2; SKCA2
• Gene ID: 3781
• mRNA Refseq: NM_021614
• Protein Refseq: NP_067627
• MIM: 605879
• UniProt ID: Q9H2S1