Recombinant Human KARS protein, His & T7-tagged

• Cat.No.: KARS-8215H
• Product Overview: Recombinant Human KARS aa. (Arg323~Arg553 (Accession # Q15046)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Arg323~Arg553
• Description: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.2kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: KARS lysyl-tRNA synthetase [ Homo sapiens (human) ]
• Official Symbol: KARS
• Synonyms: KARS; lysyl-tRNA synthetase; KRS; KARS1; KARS2; CMTRIB; DFNB89; lysine--tRNA ligase; lysRS; lysine tRNA ligase
• Gene ID: 3735
• mRNA Refseq: NM_001130089.1
• Protein Refseq: NP_001123561.1
• UniProt ID: Q15046