Recombinant Human JPH3 protein, His & T7-tagged

• Cat.No.: JPH3-1746H
• Product Overview: Recombinant Human JPH3 aa. (Met1~Leu266 (Accession # Q8WXH2)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Leu266 (Accession # Q8WXH2)
• Description: Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. CAG/CTG repeat expansion from normally 6-28 repeats to 40-59 repeats in the 3' UTR of this gene have been associated with Huntington disease-like 2 (HDL2). This gene is a member of the junctophilin gene family. Alternatively spliced transcript variants have been described for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 32.1kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 9.6

Gene Information

• Gene Name: JPH3 junctophilin 3 [ Homo sapiens (human) ]
• Official Symbol: JPH3
• Synonyms: JPH3; junctophilin 3; JP3; HDL2; JP-3; TNRC22; CAGL237; junctophilin-3; junctophilin type 3; trinucleotide repeat containing 22
• Gene ID: 57338
• mRNA Refseq: NM_020655.3
• Protein Refseq: NP_065706.2
• UniProt ID: Q8WXH2