Recombinant Human JMJD6 Protein, His-tagged

• Cat.No.: JMJD6-207H
• Product Overview: Recombinant Human JMJD6 Protein is produced by E. coli expression system. This protein is fused with a His tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: JMJD6 also known as bifunctional arginine demethylase and lysyl-hydroxylase JMJD6. JMJD6 acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as u2AF2/u2AF65 and LuC7L2, as a regulator of RNA splicing by mediating 5-hydroxylation of u2AF2/u2AF65, affecting the pre-mRNA splicing activity of u2AF2/u2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. Recombinant human JMJD6, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
• Form: Liquid, In Phosphate buffered saline (pH7.4) containing 30% glycerol, 1mM DTT
• Molecular Mass: 50.0 kDa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNHKSKK RIREAKRSAR PELKDSLDWT RHNYYESFSL SPAAVADNVE RADALQLSVE EFVERYERPY KPVVLLNAQE GWSAQEKWTL ERLKRKYRNQ KFKCGEDNDG YSVKMKMKYY IEYMESTRDD SPLYIFDSSY GEHPKRRKLL EDYKVPKFFT DDLFQYAGEK RRPPYRWFVM GPPRSGTGIH IDPLGTSAWN ALVQGHKRWC LFPTSTPRELIKVTRDEGGN QQDEAITWFN VIYPRTQLPT WPPEFKPLEI LQKPGETVFV PGGWWHVVLN LDTTIAITQN FASSTNFPVV WHKTVRGRPK LSRKWYRILK QEHPELAVLA DSVDLQESTG
• Purity: > 90% by SDS - PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 centigrade to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: JMJD6 jumonji domain containing 6 [ Homo sapiens ]
• Official Symbol: JMJD6
• Synonyms: JMJD6; jumonji domain containing 6; KIAA0585; PTDSR1; lysyl-hydroxylase JMJD6; PSR; PTDSR
• Gene ID: 23210
• mRNA Refseq: NM_001081461
• Protein Refseq: NP_001074930
• MIM: 604914
• UniProt ID: Q6NYC1