Recombinant Human JAG1 Protein, His-tagged

• Cat.No.: JAG1-2348H
• Product Overview: Recombinant Human JAG1 Protein (Gly33-Asp250) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Gly33-Asp250
• Description: The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.0 kDa
  Accurate Molecular Mass: \
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: JAG1 jagged 1 [ Homo sapiens (human) ]
• Official Symbol: JAG1
• Synonyms: JAG1; jagged 1; AGS, Alagille syndrome, JAGL1; protein jagged-1; AHD; AWS; CD339; HJ1; AGS; JAGL1; MGC104644
• Gene ID: 182
• mRNA Refseq: NM_000214
• Protein Refseq: NP_000205
• MIM: 601920
• UniProt ID: P78504