Recombinant Human ISCU, 35-167 aa, His-tagged

• Cat.No.: ISCU-945H
• Product Overview: Recombinant human ISCU protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 16.7kDa (154aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Iron-sulfur cluster assembly enzyme, also known as ISCU, is a member of the nifU family. Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. It is interact with ISCS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. ISCU-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MYHKKVVDHY ENPRNVGSLD KTSKNVGTGL VGAPACGDVM KLQIQVDEKG KIVDARFKTF GCGSAIASSS LATEWVKGKT VEEALTIKNT DIAKELCLPP VKLHCSMLAE DAIKAALADY KLKQEPKKGE AEKK.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ISCU iron-sulfur cluster scaffold homolog (E. coli) [ Homo sapiens ]
• Synonyms: iron-sulfur cluster scaffold homolog (E. coli);iron-sulfur cluster assembly enzyme ISCU, mitochondrial; nifU-like protein; nitrogen fixation cluster-like; NifU-like N-terminal domain containing; IscU iron-sulfur cluster scaffold homolog; nifU-like N-terminal domain-containing protein; HML; ISU2; NIFU; NIFUN; hnifU; MGC74517; 2310020H20Rik; ISCU; NifU-like protein; NifU-like N-terminal domain-containing protein; IscU iron-sulfur cluster scaffold homolog (E. coli)
• Gene ID: 23479
• mRNA Refseq: NM_014301
• Protein Refseq: NP_055116
• MIM: 611911
• UniProt ID: Q9H1K1
• Chromosome Location: 12q24.1
• Function: iron ion binding; iron-sulfur cluster binding; metal ion binding; protein binding; protein complex scaffold