Recombinant Human IRF3 protein, His/T7-tagged

• Cat.No.: IRF3-61H
• Product Overview: Recombinant Human IRF3(Met1~Lys360) fused with His/T7 tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Lys360
• Description: This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Form: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Molecular Mass: 43.8kDa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MGTPKPRILP WLVSQLDLGQ LEGVAWVNKS RTRFRIPWKH GLRQDAQQED FGIFQAWAEA TGAYVPGRDK PDLPTWKRNF RSALNRKEGL RLAEDRSKDP HDPHKIYEFV NSGVGDFSQP DTSPDTNGGG STSDTQEDIL DELLGNMVLA PLPDPGPPSL AVAPEPCPQP LRSPSLDNPT PFPNLGPSEN PLKRLLVPGE EWEFEVTAFY RGRQVFQQTI SCPEGLRLVG SEVGDRTLPG WPVTLPDPGM SLTDRGVMSY VRHVLSCLGG GLALWRAGQW LWAQRLGHCH TYWAVSEELL PNSGHGPDGE VPKDKEGGVF DLGPFIVDLI TFTEGSGRSP RYALWFCVGE SWPQDQPWTK
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: IRF3 interferon regulatory factor 3 [ Homo sapiens ]
• Official Symbol: IRF3
• Synonyms: IRF3; interferon regulatory factor 3
• Gene ID: 3661
• mRNA Refseq: NM_001197128
• Protein Refseq: NP_001184057
• MIM: 603734
• UniProt ID: Q14653
• Chromosome Location: 19q13.3-q13.4
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Apoptosis, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem
• Function: DNA binding; identical protein binding; protein binding; protein homodimerization activity; regulatory region DNA binding; sequence-specific DNA binding transcription factor activity; transcription cofactor activity; transcription regulatory region DNA binding