Recombinant Human Interleukin 9, HQ-tagged

• Cat.No.: IL9-5342H
• Product Overview: Recombinant humanIL9 contains 127 amino acids and a 16 aa HQ tag. Total length is 143 aa.

Specification

• Cat. No.: IL9-5342H
• Description: Interleukin 9, alsoknown as IL9, is a cytokine (cell signalling molecule) belonging to the groupof interleukins. The protein encoded by this gene is a cytokine produced byT-cells and specifically by CD4+ helper cells that acts as a regulator of avariety of hematopoietic cells. This cytokine stimulates cell proliferationand prevents apoptosis. It functions through the interleukin-9 receptor(IL9R), which activates different signal transducer and activator (STAT)proteins and thus connects this cytokine to various biological processes. Thegene encoding this cytokine has been identified as a candidate gene forasthma.
• Source: Hordeum vulgare(barley grain)
• Species: Human
• Form: Lyophilized from a0.2 µm sterile filtered solution of PBS, pH 7.2.
• Molecular Weight: Predicted MW 16.2kDa. The glycosylated recombinant protein migrates as 3 bands with anapparent MW 20, 22 and 24 kDa in SDS-PAGE.
• Purity: >95% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction.
• Endotoxin Level: < 0.005 ng perµg of product (0.05 EU/µg) as measured by turbidimetric kinetic assay.
• Biological Activity: Bioactivity is assayed by measuring the dosedependent effect of human IL9 on proliferation of mouse TS1h9RA3 cell line,transfected with human IL9 receptor. The ED50 for a typical batch of recombinanthuman IL9, is 0.1 ng/ml.
• Reconstitution: Centrifuge vial prior to opening. First add steriledistilled water to the vial to fully solubilize the protein to aconcentration not less than 100 µg/ml. After complete solubilization of theprotein, it can be further diluted to other aqueous solutions.
• Storage: The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: IL9

Gene Information

• Gene Name: IL9 interleukin 9 [ Homosapiens ]
• Synonyms: IL9; interleukin 9;P40; HP40; IL-9; interleukin-9; Cytokine P40; cytokine P40; p40 cytokine; OTTHUMP00000159423;T-cell growth factor p40; p40 T-cell and mast cell growth factor; homolog ofmouse T cell and mast cell growth factor 40
• Gene ID: 3578
• mRNA Refseq: NM_000590
• Protein Refseq: NP_000581
• MIM: 146931
• UniProt ID: P15248
• Chromosome Location: 5q31.1
• Pathway: Asthma;Cytokine-cytokine receptor interaction; IL-9 Signaling Pathway; Jak-STATsignaling pathway
• Function: cytokine activity;growth factor activity; interleukin-9 receptor binding