Recombinant Human Interleukin 7, 152aa

• Cat.No.: IL7-66H
• Product Overview: Recombinant Human Interleukin-7 produced inYeastis a single, glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17.4 kDa. The IL-7 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: S.Cerevisiae
• Tag: Non
• Description: IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V (D) J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Cys-Asp-Ile-Glu.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM phosphate buffer.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin-7 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED₅₀as determined by the dose-dependent stimulation of thymidine uptake by murine pre-B cell line 2E8 is < 0.5 ng/mL, corresponding to a specific activity of > 2 x 10⁶IU/mg.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.
• Storage: Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL7 should be stored at 4℃between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IL7 interleukin 7 [ Homo sapiens ]
• Synonyms: IL7; interleukin 7; IL-7; Interleukin-7;IL7 nirs variant 6
• Gene ID: 3574
• mRNA Refseq: NM_000880
• Protein Refseq: NP_000871
• UniProt ID: P13232
• Chromosome Location: 8q12-q13
• MIM: 146660
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-7 receptor binding; protein binding