Recombinant Human Interleukin 5 receptor, Alpha, 308aa, Fc Chimera

• Cat.No.: IL5RA-126H
• Product Overview: Recombinant Human interleukin 5 receptor, alpha encoding the signal peptide and extracellular domain of human Interleukin 5 receptor alpha chain (aa 1-327) was expressed in modifiedhuman 293 cells. It was fused to the Fc region of human IgG1 (aa 93-330).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-327 a.a.
• Description: Interleukin 5 (IL-5) plays an important role in B cell development by inducing the terminal differentiation of late-developing B cells. Additionally, IL-5 regulates the differentiation, survival and degranulation of eosinophils. IL-5 is expressed predominately by T-lymphocytes and mast cells, and to a lesser extent by eosinophils, natural killer cells and endothelial cells.
• Amino Acid Sequence: DLLPDEKISLLPPVNF TIKVTGLAQVLL QWKP NPDQ EQRN VNLEYQVKIN APKEDDYET RITES KCVTIL HKGFSASVRTILQNDHSLLASSWASA EL HAPPGSPG TSIVNLTCTTN T TEDNYSRLRSY QVSLHCTWLVG TDAPEDTQYFL YYR YGS WTEEC QEYSKDTLGRN IACWFPRTFILS KG RD WLAVLV NGSSKHSAIRP FDQL FAL HAID QINPP LNVTA EIEGTRLSIQW EKPVSAFPIHC FDY EVKIHNTR NGYL QIEKLMT NAFISIIDDLSKY DVQVRAAV SSMCREAG LWSEWSQ RIPKVDKKV EP KSCDKT HTCPPCPA PELLGGPS VFLFPPKP KDTLMISRTPE VTCVVVD VS HEDPE VK FNWYV DGVEVHN AKTKPREEQYNST YRVVSVLT VLHQDWL NG KE YKCRVSNKALPAPI EK TIS KA K GQPREPQ VYTLPPSR DELT KNQ VSLTC LVK GFYPSD IAVEWESNG QPENNYK TTP PVL DS DGS FFLY SKLT VDK SRW QQG NVFSCSVMHEALHNHYTQKSLSLSPGK.
• Molecular Mass: Under reducing conditions IL-5R alpha (308aa)-Fc Chimera migrates as a broad band between 70 and 80 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-5R alpha (308aa)-Fc Chimera that has a predicted monomeric molecular mass of 62.1 kDa.
• PI: IL-5R alpha (308aa)-Fc Chimeraseparates into a number of glycoforms with a pI between 5.8 and 7.0 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-5R alpha (308aa)-Fc Chimera that has a predicted pI of 6.34.
• % Carbohydrate: Purified IL-5R alpha (308aa)-Fc Chimera consists of 10-25% carbohydrate by weight.
• Glycosylation: IL-5R alpha (308aa)-Fc Chimerahas N-linked and O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Gene Information

• Gene Name: IL5RA interleukin 5 receptor, alpha [ Homo sapiens ]
• Synonyms: IL5RA; interleukin 5 receptor, alpha; CD125; CDw125; HSIL5R3; IL5R; MGC26560; CD125 antigen; OTTHUMP00000115373; OTTHUMP00000206893; OTTHUMP00000206896; interleukin 5 receptor type 3; interleukin-5 receptor alpha chain; interleukin-5 receptor alpha subunit; OTTHUMP00000115257; IL-5R-alpha
• Gene ID: 3568
• mRNA Refseq: NM_000564
• Protein Refseq: NP_000555
• MIM: 147851
• UniProt ID: Q01344
• Chromosome Location: 3p26-p24
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Hematopoietic cell lineage
• Function: interleukin-5 receptor activity; protein binding