Recombinant Human Interleukin 27 Receptor, Alpha, Fc Chimera

• Cat.No.: IL27RA-781H
• Product Overview: Recombinant HumanIL27RA encoding the extracellular domain (Met1-Arg514) of human IL27Rα (NP_004834.1) pro-form was produced inE.coli.It was expressed with the C-terminal fused Fc region of human IgG1.

Specification

• Cat. No.: IL27RA-781H
• Description: L27Rα (IL27R), also well known as WSX-1 and TCCR, is a type I cytokine receptor that functions only as the ligand binding component of the receptor for IL27, and together with the glycoprotein 130 (gp130) coreceptor constitutes a functional signal-transducing receptor mediating the IL27 effects. Neither receptor chain alone is sufficient independent of the other. As a member of the IL6/IL12 family of cytokines, IL27 is not only involved in the development of Th1-type adaptive immune response, but also acts on cells of the innate immune system. In addition, it has been demonstrated that IL27Rα, the single component of a heterodimeric cytokine receptor, can aberrantly activate signaling pathways and transform hematopoietic cells in a JAK-dependent manner in acute myeloid leukemia (AML). Accordingly, IL27Rα may play an unappreciated role in various myeloproliferative disorders (MPDs) and be identified as a future therapeutic target for this disease.
• Molecular Mass: The recombinant human IL27Rα/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 722 amino acids and has a predicted molecular mass of 79kDa. As a result of glycosylation, the rh IL27Rα/Fc monomer migrates as an approximately 105-115kDa band in SDS-PAGE under reducing conditions.
• Source: Escherichia Coli.
• Predicted N Terminal: Gln 21.
• Endotoxin: <1.0EU per 1μg cytokine as determined by the LAL method.
• Formulation: Supplied as a 0.2μm filtered solution of PBS, pH7.2.
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Purity: >95%, as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt -70°C.
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Protein length: 1-514 a.a.

Gene Information

• Gene Name: IL27RA interleukin 27 receptor, alpha [ Homo sapiens ]
• Synonyms: IL27RA; interleukin 27 receptor, alpha; CRL1; TCCR; WSX1; IL27R; zcytor1; WSX-1; IL-27R; class I cytokine receptor; T-cell cytokine receptor type 1; Interleukin-27 receptor subunit alpha; IL-27R-alpha; WSX-1; Type I T-cell cytokine receptor; Protein CRL1
• Gene ID: 9466
• mRNA Refseq: NM_004843
• Protein Refseq: NP_004834
• MIM: 605350
• UniProt ID: Q6UWB1
• Chromosome Location: 19p13.11
• Function: interleukin-27 receptor activity; protein binding; transmembrane receptor activity