Recombinant Human Interleukin-17

• Cat.No.: IL17A-26H
• Product Overview: Recombinant human IL-17 produced inE.coliis a 31.0kDa disulfide-linked homodimer of two 136 amino acid polypeptide chains.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 132 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells.
• Purity: >95% as determined by SDS-PAGE.
• Formulation: Sterile filtered lyophilized powder, with 20mM Citrate, 0.1M NaCl, pH4.5.
• Specific Activity: The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line was found to be approximately 2ng/ml, corresponding to a specific activity of 5.0 x 10⁵IU/mg.
• Endotoxin: Less than 1 IEU/µg determined by LAL test.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-17 in sterile 18MΩ-H₂O containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 100ug/ml of the cytokine.
• Storage: Lyophilized rHuIL-17 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-17 should be stored at 4°C between 2-7 days and for future use below -18°C. Aliquot to avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: IL17A
• Synonyms: IL17; CTLA-8; IL-17; IL-17A; Cytotoxic T-lymphocyte-associated antigen 8; Interleukin-17A, CTLA8; Interleukin-17APrecursor; IL17A; interleukin17A
• Gene ID: 3605
• mRNA Refseq: NM_002190
• Protein Refseq: NP_002181
• MIM: 603149
• UniProt ID: Q16552
• Chromosome Location: 6p12
• Pathway: Cytokine-cytokine receptor interaction
• Function: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.