Active Recombinant Rat Interleukin-17AF Heterodimer

• Cat.No.: Il17a-492R
• Product Overview: Recombinant rat IL-17A/F produced in E.coli is a heterodimeric non-glycosylated polypeptide comprised of one monomeric subunit each of r-IL-17A and r-IL-17F. The dimer consists of 271 amino acids, with an approximate molecular weight of 30.7 kDa.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: Heterodimeric interleukin-17A/F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Recent IL-17A/IL-17F co-expression studies in HEK-293 cells revealed the presence of a biologically active heterodimer, IL-17A/F. Activated CD4+ T cells have also been shown to produce human IL-17A/F. Activity studies reveal hIL-17A to be the most potent, followed by hIL-17A/F and hIL-17F 100 fold lower than hIL-17A. IL-17A/F is thought to induce neutrophilia and chemokine expression via the IL-17RA receptor.
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 98% as determined by: Reducing and non-reducing SDS-PAGE.
• Bio-activity: In process.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 97% as determined by: Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MAVLIPQSSV CPNAEANNFL QNVKVNLKVL NSLSSKASSR RPSDYLNRST SPWTLSRNED PDRYPSVIWE AQCRHQRCVN AEGKLDHHMN SVLIQQEILV LKREPEKCPF TFRVEKMLVG VGCTCVSSIV RHAS MARRNPKVGL SALQKAGNCP PLEDNSVRVD IRIFNQNQGI SVPRDFQNRS SSPWD YNITR DPDRFPSEIA EAQCRHSGCI NAQGQEDGSM NSV PIQQEIL VLRREPQGCS NSFRLEKMLI KVGCTCVTPI VHH AA
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: Il17a interleukin 17A [ Rattus norvegicus ]
• Official Symbol: Il17a
• Synonyms: Il17a; interleukin 17A; Il17; Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• Gene ID: 301289
• mRNA Refseq: NM_001106897
• Protein Refseq: NP_001100367
• Chromosome Location: Q61453
• Function: cytokine activity