Recombinant Human Interferon, Alpha 2

• Cat.No.: IFNA2-59H
• Product Overview: Recombinant human intefferon α2a (rhIFNα2a) produced inSaccharomyces cerevisiaeis a non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 19.2kDa. It is manufactured through Chromatography in the GMP facilities.

Specification

• Species: Human
• Source: S.Cerevisiae
• Tag: Non
• Description: At least 23 different variants of Interferon-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxyl-terminal end.
• Amino Acid Sequence: The sequence of the first fifteen N-terminal amino acid residues was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg-Arg-Thr-Leu.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder,or Sterile filtered liquid accoriding to the client's demands.
• Purity: Greater than 97.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized or liquid containing Sodium phosphate dibasic, sodium phosphate monobasic and HSA, pH7.0±0.5.
• Solubility: It is recommended to reconstitute the lyophilized rhIFN-α2a in water for injection not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Before adding the carrier HSA protein, rhIFN-α2a Protein quantitation was carried out by Lowry method. rhIFN-α2a protein concentration is usually between 1.5 ~2.0mg/mL according to the client's demands.
• Endotoxin: Less than 1.0 EU/mg of rh-IFN-α 2a concentrated solution (LAL test).
• Biological Activity: The specific activity, determined by the dose-dependent proliferation of WISH (heteroploid human amnion cell line) in presence of VSV ( vesicular stomatitis virus), was found to be no less than 1.8 × 10⁸ IU/ mL.
• Residual Extraneous DNA: Dot blot hybridization method with DIG-labeled DNA probe, it shall be not more than 10ng/0.1mg protein.
• Residual Host Bacterial Proteins: ELISA method is used; the residual host bacterial proteins shall be not more than 0.050 % of total proteins.
• Stability: Lyophilized rhIFN-α2a although stable at 30°C for 6 months, should be stored desiccated below 4°C no more than 3 years. Upon reconstitution it should be stored at 4°C between 3-4 days and for future use below -18°C or more low. Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: IFNA2 interferon, alpha 2 [ Homo sapiens ]
• Synonyms: IFNA2; Interferon, Alpha 2; IFNA; INFA2; MGC125764; MGC125765; Interferon alpha A; Alpha-2a interferon; Interferon alpha 2b; OTTHUMP00000021143; Interferon alpha-A; LeIF A; Interferon, alpha-2
• Gene ID: 3440
• mRNA Refseq: NM_000605
• Protein Refseq: NP_000596
• MIM: 147562
• UniProt ID: P01563
• Chromosome Location: 9p22
• Pathway: Antigen processing and presentation; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Regulation of autophagy;Toll-like receptor signaling pathway
• Function: interferon-alpha/beta receptor binding