Recombinant Human Insulin-Like Growth Factor 1 Receptor

• Cat.No.: IGF1R-663H
• Product Overview: Recombinant IGF1R is adisulfide-linked monomeric protein consisting of 71 amino acid residuesubunits. IGF1R migrates as an approximately 8 kDa protein under non-reducingand reducing conditions in SDS-PAGE, and was lyophilized from a 0.2 μmfiltered PBS solution, pH 7.4.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: IGF1R,also known as somatomedin C, is the dominant effector of growth hormone andis structurally homologous to proinsulin. Human IGF1R is synthesized as twoprecursor isoforms with N- and alternate C- terminal propeptides. Theseisoforms are differentially expressed by various tissues. The 7.6 kDa matureIGF-I is identical between isoforms and is generated by proteolytic removalof the N- and C- terminal regions. Mature human IGF1R shares 94% and 96% aasequence identity with mouse and rat IGF-I, respectively, and exhibitscross-species activity. It shares 64% aa sequence identity with mature humanIGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I isproduced by many other tissues in which it has paracrine effects.
• Purity: >95%, asdetermined by SDS-PAGE and HPLC.
• Sequence: MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGPETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSARSVRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM
• Molecular Weight: Nativehuman Insulin Growth Factor-1 is generated by the proteolytic removal of thesignal peptide and propeptide, the molecule has a calculated molecular massof approximately 8 kDa.
• Biological Activity: TheED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 1.0ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
• Endotoxin: Endotoxin contentwas assayed using a LAL gel clot method. Endotoxin level was found to be lessthan 0.1 ng/µg(1EU/µg).
• Reconstitution: A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Storage: The lyophilized protein is stable forat least 2 years from date of receipt at -20°C. Upon reconstitution, thiscytokine can be stored in working aliquots at 2°C - 8°C for one month, or at-20°C for six months, with a carrier protein without detectable loss ofactivity. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: IGF1R
• Pathways: Adherens junction;Apoptosis; Endochondral Ossification; Endocytosis; Glioma; Glioma; IGF1pathway; Id Signaling Pathway; Insulin Signaling; Integrins in angiogenesis; Long-termdepression; Melanoma; Pathways in cancer; Pathways in cancer; Prostatecancer; Senescence and Autophagy

Gene Information

• Gene Name: IGF1Rinsulin-like growth factor 1 receptor [ Homo sapiens ]
• Synonyms: IGF1R; insulin-like growth factor 1receptor; IGFR; CD221; IGFIR; JTK13; MGC18216; MGC142170;MGC142172; insulin-like growth factor 1 receptor; IGF-I receptor;OTTHUMP00000194784; soluble IGF1R variant 1; soluble IGF1R variant 2;insulin-like growth factor I receptor
• Gene ID: 3480
• mRNA Refseq: NM_000875
• Protein Refseq: NP_000866
• MIM: 147370
• UniProt ID: P08069
• Chromosome Location: 15q26
• Function: ATPbinding; identical protein binding; insulin binding; insulin receptorbinding; insulin receptor substrate binding; insulin-like growthfactor-activated receptor activity; metal ion binding; nucleotide binding;phosphatidylinositol 3-kinase binding; protein binding; protein tyrosinekinase activity; receptor activity