Recombinant Human INPP5D 293 Cell Lysate

• Cat.No.: INPP5D-5198HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for inositol polyphosphate-5-phosphatase, 145kDa (INPP5D), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: INPP5D inositol polyphosphate-5-phosphatase, 145kDa [ Homo sapiens ]
• Official Symbol: INPP5D
• Synonyms: INPP5D; inositol polyphosphate-5-phosphatase, 145kDa; inositol polyphosphate 5 phosphatase, 145kD; phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; hp51CN; SHIP; SHIP-1; p150Ship; SH2 domain-containing inositol phosphatase 1; SH2 containing inositol phosphatase, isoform b; SH2 domain-containing inositol 5-phosphatase 1; SH2 domain-containing inositol-5-phosphatase 1; inositol polyphosphate-5-phosphatase of 145 kDa; signaling inositol polyphosphate 5 phosphatase SIP-145; phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1; SHIP1; SIP-145; MGC104855; MGC142140; MGC142142
• Gene ID: 3635
• mRNA Refseq: NM_001017915
• Protein Refseq: NP_001017915
• MIM: 601582
• UniProt ID: Q92835
• Chromosome Location: 2q37.1
• Pathway: 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian), organism-specific biosystem; 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian), conserved biosystem; 3-phosphoinositide degradation, organism-specific biosystem; 3-phosphoinositide degradation, conserved biosystem; Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem
• Function: PTB domain binding; SH3 domain binding; hydrolase activity; inositol-4,5-bisphosphate 5-phosphatase activity; inositol-polyphosphate 5-phosphatase activity; phosphatidylinositol trisphosphate phosphatase activity; protein binding