Recombinant human influenza B virus Hemagglutinin protein, His tagged

• Cat.No.: HA-1175I
• Product Overview: C-terminal 6×His tagged ectodomain of human influenza B virus (B/Florida/4/2006) HA protein (amino acid 16-546) (Genebank No. ACA33493)

Specification

• Species: Influenza B virus (B/Florida/4/2006)
• Source: HEK293
• Tag: His
• Protein Length: 16-546 aa
• Description: Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
• Tag: C-6×His
• AA Sequence: DRICTGITSSNSPHVVKTATQGEVNVTGVIPLTTTPTKSYFANLKGTRTRGKLCPDCLNCTDLDVALGRPMCVGTTPSAKASILHEVKPVTSGCFPIMHDRTKIRQLPNLLRGYENIRLSTQNVIDAKAPGGPYRLGTSGSCPNATSKSGFFATMAWAVPKDNNKNATNPLTVEVPYICTEGEDQITVWGFHSDDKTQMKNLYGDSNPQKFTSSANGVTTHYVSQIGSFPDQTEDGGLPQSGRIVVDYMMQKPGTGTIVYQRGVLLPQKVWCASGRSKVIKGSLPLIGEADCLHEKYGGLNKSKPYYTGEHAKAIGNCPIWVKTPLKLANGTKYRPPAKLLKERGFFGAIAGFLEGGWEGMIAGWHGYTSHGAHGVAVAADKSTQEAINKITKNLNSLSELEVKNLQRLSGAMDELHNEILELDEKVDDLRADTISSQIELAVLLSNEGIINSEDEHLLALERKLKKMLGPSAVEIGNGCFETKHKCNQTCLDRIAAGTFNAGEFSLPFDSLNITAASLNDDGLDNH
• Endotoxin: <0.01 EU/μg of the protein by LAL test
• Purity: > 95% by SDS-PAGE
• Applications: Western Blot standard (WB), ELISA (EIA)
• Storage: Store at -20 centigrade; Stable for 6-months from the date of receipt when kept at 4 centigrade. Non-hazardous.
• Storage Buffer: PBS
• Concentration: 1 μg/μL

Gene Information

• Official Symbol: HA
• Synonyms: Hemagglutinin; HA; B1AG46_9INFB