Recombinant H2N2 (A/Canada/720/2005) HA Protein, His-tagged

• Cat.No.: HA-342V
• Product Overview: Recombinant extracellular domain (Met 1-Gln 516) of the influenza hemagglutinin (A/Canada/720/2005 (H2N2)) (AAY28987.1) (HA1+HA2, uncleaved) was fused with a C-terminal polyhistidine tag.

Specification

• Species: H2N2
• Source: HEK293
• Tag: His
• Protein Length: 1-516 a.a.
• Predicted N Terminal: Asp 16
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant influenza A H2N2 HA (A/Canada/720/2005 (H2N2)) comprises 521 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.